For the 2-step, there are some tweaks that typically need to be made in the chip. Normally the alcohol does not need to be on, but if it works ok with it on, you can run it. The problem with keeping the alky off, is it might not ramp up fast enough once you launch. WOT closed loop only enables over about 10psi boost, so it depends on what boost you launch at, but generally we don't want closed loop when on the 2-step.
Usually people will try it out as is, then if there's problems, we modify the chip programming.